RESUMEN
OBJECTIVE: To study the extraction technology of polysaccharide from Penthorum chinense. METHODS: Single factor test and orthogonal experiment design methods L9 (3(3)) were applied to analyze the influence of solid-liquid rate, extraction temperature and time on the extraction percent of polysaccharide from Penthorum chinense. RESULTS: The temperature significantly affected the extraction percent of the soluble polysaccharide, and the optimum conditions for the extraction technology were as follows: solid-liquid rate at 1:15, temperature at 95 degrees C, time for 3h. Under this condition, the extraction percent of polysaccharide from Penthorum chinense was 1.12%, RSD = 1.21%. CONCLUSION: The research can provide a theoretical foundation for further development and utilization of polysaccharide from Penthorum chinense.
Asunto(s)
Plantas Medicinales/química , Polisacáridos/aislamiento & purificación , Saxifragaceae/química , Tecnología Farmacéutica/métodos , Análisis de Varianza , Medicamentos Herbarios Chinos/química , Etanol , Polisacáridos/análisis , Espectrofotometría Ultravioleta , Temperatura , Factores de TiempoRESUMEN
Four parameters, three hormones and sucrose, at seven concentrations, were designed for shoot proliferation of Penthorum chinense by uniform design. The obtained data were used for building two quadratic polynomial equations by partial least square to determine optimum concentrations of four factors. Experiments for verification confirmed that no significant difference existed between the predicted and the validated values in shoot number and length based on all inoculated explants.
